Potency Lignocellulose Degrading Bacteria Isolated from Bali Cattle Rumen Content Waste and Termites as Nonconventional Waste Degrader

A research has been carried out to evaluate the potency of lignocellulose degrading bacteria isolated from bali cattle rumen content waste and termites.Those animals were chosen sinceit hasbeen consumedfor its low quality crude fiber as the main energy sources. Lignocellulose degrading bacteria were isolated by Hungate selective media,using lignin (tannic acid), xylan, and cellulose as selective substrates. The best lignocellulose degrading bacteria then was determined by enzyme activity by isolate. It showed that lignocellulose degrading bacteria could be found in Bali cattle rumen content waste and termites. In bali cattle rumen content waste found 4 isolate lignocellulolytic, 5 isolatecellulolytic, and 5 isolatexilanolytic bacteria. Even though from termites found 8 isolate cellulolytic and 1 isolate xilanolytic. Enzyme activity evaluation showed first and second highest lignocellulolytic, cellulolytic, and xylanolytic activities from bali cattle rumen content waste isolates were reached by BCR Ligsll 2and isolate BCR Ligsll 4, isolate BCR CMC 2-2and isolate BCR CMC 2-3, BCR Xy 2 and BCR Xy 3. Meanwhile from termites first and second highest cellulolytic, and xylanolytic activities were reached by BR CMC 3-7 and BR CMC 3-2, isolate BR Xy. It can be concluded that nine isolate bacteria has highest enzyme activity chosen as nonconventional waste degrader for bali cattle feed production. Key word: Bacteria, Lignocellulose, Nonconventional Waste, Rumen Content Waste and termites